Starting at: $74.00
| SKU | Package Size | Price | Quantity | Add to Cart |
|---|---|---|---|---|
| IX03139-1 | 1mg | Starting at: $1,373.00 | ||
| IX03139-10 | 10ug | Starting at: $74.00 | ||
| IX03139-50 | 50ug | Starting at: $199.00 |
Starting at: $699.00
Starting at: $1,012.00
Overview
| Synonyms | Ciliary Neurotrophic Factor |
| Description | Ciliary Neurotrophic Factor (CNTF) is a cytokine belonging to the Interleukin 6 (IL-6) family, which also includes IL-6, Oncostatin M, Leukemia Inhibitory Factor (LIF), and Cardiotrophin-1. Structurally, CNTF resembles a four-helix bundle composition, similar to the other members of the IL-6 family. The receptor for CNTF is composed of three parts: a gp130-like subunit common in the IL-6 receptor family, a LIF Receptor β subunit, and a CNTF specific α receptor subunit. Upon binding to the CNTF, the β subunit of the CNTF receptor will undergo tyrosine phosphorylation, and activate the intracellular JAK/STAT pathway. The main function of CNTF in vivo is to promote the differentiation and survival of a variety of neurons and glial cells, including sympathetic precursor cells and spinal motor neurons. Recombinant human Ciliary Neurotrophic Factor (rhCNTF) produced in E. coli is a single non-glycosylated polypeptide chain containing 199 amino acids. A fully biologically active molecule, rhCNTF has a molecular mass of 22.8 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques. |
| Accession No | P26441 |
| Species | Human |
| Source | E. coli |
| Biological Activity | ED50 < 200 ng/ml, measured cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 5×103 units/mg. |
| Sequence |
AFTEHSPLTP HRRDLCSRSI WLARKIRSDL TALTESYVKH
QGLNKNINLD SADGMPVAST DQWSELTEAE RLQENLQAYR TFHVLLARLL EDQQVHFTPT EGDFHQAIHT LLLQVAAFAY QIEELMILLE YKIPRNEADG MPINVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRFISS HQTGIPARGS HYIANNKKM |
Properties
| Measured Molecular Weight | 22.8 kDa, observed by reducing SDS-PAGE. |
| Purity | > 95% as analyzed by SDS-PAGE and HPLC. |
| Formulation | Lyophilized after extensive dialysis against PBS. |
| Reconstitution | Reconstituted in ddH2O or PBS at 100 μg/ml. |
| Endotoxin Level | < 0.2 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant human Ciliary Neurotrophic Factor (rhCNTF) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhCNTF remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
| Note | For research use only |