Starting at: $49.00
| SKU | Package Size | Price | Quantity | Add to Cart |
|---|---|---|---|---|
| IX03166-1 | 1mg | Starting at: $749.00 | ||
| IX03166-10 | 10ug | Starting at: $49.00 | ||
| IX03166-50 | 50ug | Starting at: $107.00 |
Starting at: $699.00
Starting at: $1,012.00
Overview
| Synonyms | FGF-2; BFGF; FGFB; HBGF-2 |
| Description | Fibroblast Growth Factor-basic (FGF-basic), also known as FGF-2, is a pleiotropic cytokine and one of the prototypic members of the heparin-binding FGF family. Like other FGF family members, FGF-basic has the β trefoil structure. In vivo, FGF-basic is produced by a variety of cells, including cardiomycotes, fibroblasts, and vascular cells. FGF-basic regulates a variety of processes including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation and wound healing. FGF-basic can be tumorigenic due to its role in angiogenesis and blood vessel remodeling. The angiogenic effects of FGF-basic can produce beneficial cardioprotection during acute heart injury. Recombinant human Fibroblast Growth Factor-basic (146 a.a.) (rhFGF-basic) produced in E. coli is a single non-glycosylated polypeptide chain containing 146 amino acids. A fully biologically active molecule, rhFGF-basic has a molecular mass of 16.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques. |
| Accession No | P09038 |
| Species | Human |
| Source | E. coli |
| Biological Activity | ED50 < 0.25 ng/mL, measured by the cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 4 × 106 units/mg. |
| Sequence |
PALPEDGGSG AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV
DGVREKSDPH IKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTD ECFFFERLES NNYNTYRSRK YTSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS |
Properties
| Measured Molecular Weight | 16.4 kDa, observed by reducing SDS-PAGE. |
| Purity | > 95% by SDS-PAGE analysis. |
| Formulation | Lyophilized after extensive dialysis against PBS. |
| Reconstitution | Reconstituted in ddH2O at 50 μg/mL. |
| Endotoxin Level | < 0.2 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant human Fibroblast Growth Factor-basic (146 a.a.) (rhFGF-basic) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhFGF-basic remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
| Note | For research use only |