|Synonyms||FGF-2; BFGF; FGFB; HBGF-2|
|Description||Fibroblast Growth Factor-basic (FGF-basic), also known as FGF-2, is a pleiotropic cytokine and one of the prototypic members of the heparin-binding FGF family. Like other FGF family members, FGF-basic has the β trefoil structure. In vivo, FGF-basic is produced by a variety of cells, including cardiomycotes, fibroblasts, and vascular cells. FGF-basic regulates a variety of processes including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation and wound healing. FGF-basic can be tumorigenic due to its role in angiogenesis and blood vessel remodeling. The angiogenic effects of FGF-basic can produce beneficial cardioprotection during acute heart injury.
Recombinant human Fibroblast Growth Factor-basic (146 a.a.) (rhFGF-basic) produced in E. coli is a single non-glycosylated polypeptide chain containing 146 amino acids. A fully biologically active molecule, rhFGF-basic has a molecular mass of 16.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques.
|Biological Activity||ED50 < 0.25 ng/mL, measured by the cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 4 × 106 units/mg.|
PALPEDGGSG AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV
DGVREKSDPH IKLQLQAEER GVVSIKGVCA NRYLAMKEDG
RLLASKCVTD ECFFFERLES NNYNTYRSRK YTSWYVALKR
TGQYKLGSKT GPGQKAILFL PMSAKS
|Measured Molecular Weight||16.4 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE analysis.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O at 50 μg/mL.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Fibroblast Growth Factor-basic (146 a.a.) (rhFGF-basic) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhFGF-basic remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|