Starting at: $107.00
| SKU | Package Size | Price | Quantity | Add to Cart |
|---|---|---|---|---|
| IX02695-1 | 1mg | Starting at: $2,621.00 | ||
| IX02695-10 | 10ug | Starting at: $107.00 | ||
| IX02695-50 | 50ug | Starting at: $344.00 |
Starting at: $699.00
Starting at: $1,012.00
Overview
| Synonyms | Granulocyte Macrophage Colony Stimulating Factor, CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim, MGC131935, MGC138897 |
| Description | Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF), a hematopoietic growth factor, is mainly involved in granulopoiesis and monocytopoiesis. It is produced by T-cells and macrophages in response to antigens, and by endothelial cells and fibroblasts following induction of variouscytokines [1]. A monomeric protein of 127 amino acidswith six glycosylation sites and two intra disulfide bonds [2,3], glycosylated and non-glycosylated hGM-CSFs show similar biological activities [4].Other than its connection to the growth and development of granulocytes and macrophages, it is also indispensable for the proliferation of erythroid and megakaryocytic cells [5]. Recombinant human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) produced in E. coli is a non-glycosylated polypeptide. A fully biologically active molecule, rhGM-CSF has a molecular mass of 14 kDa analyzed by SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques. |
| Species | Human |
| Source | E. coli |
| Biological Activity | ED50 < 0.5 ng/ml, measured by proliferation assay of TF-1 cells, corresponding to a specific activity of >2.0 x 106units/mg |
| Sequence |
MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET
VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE |
Properties
| Measured Molecular Weight | 14 kDa, observed by SDS-PAGE. |
| Purity | >95 % as analyzed by SDS-PAGE. |
| Formulation | Lyophilized after extensive dialysis against 10 mM PB, pH7.0. |
| Reconstitution | Reconstituted in ddH2O at 100μg/ml. |
| Endotoxin Level | <1 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) remains stable up to 12 months at lower than -70°C from date of receipt. Upon reconstitution, rhGM-CSF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C. |
| Note | For research use only |