|Synonyms||GMCSF,Granulocyte/Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, pluripoietin-alpha|
|Description||Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine and other immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor that activates effector functions of granulocytes, monocytes/macrophages and eosinophils.
Recombinant Mouse GM-CSF produced in E.coli is a single non-glycosylated polypeptide chain containing 125 amino acids. A fully biologically active molecule, rmGM-CSF has a molecular mass of 14.3 kD analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.
|Biological Activity||ED50 < 5 pg/ml, measured in a cell proliferation assay using mouse FDC-P1 cells, corresponding to a specific activity of >2 x 108 units/mg.|
Ala18-Lys141 (Accession #: Q14AD9), with an N-terminal Met.
|Measured Molecular Weight||14.3 kDa, observed by reducing SDS-PAGE.|
|Purity||> 98% as analyzed by SDS-PAGE&HPLC.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant Mouse GM-CSF remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Mouse GM-CSF should be stable up to 1 week at 4°C or up to 3 months at -20°C.|
|Note||For research use only|