Starting at: $82.00
| SKU | Package Size | Price | Quantity | Add to Cart |
|---|---|---|---|---|
| IX03300-1 | 1mg | Starting at: $1,872.00 | ||
| IX03300-10 | 10ug | Starting at: $82.00 | ||
| IX03300-50 | 50ug | Starting at: $245.00 |
Starting at: $699.00
Starting at: $1,012.00
Overview
| Synonyms | GMCSF,Granulocyte/Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, pluripoietin-alpha |
| Description | Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine and other immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor that activates effector functions of granulocytes, monocytes/macrophages and eosinophils. Recombinant Mouse GM-CSF produced in E.coli is a single non-glycosylated polypeptide chain containing 125 amino acids. A fully biologically active molecule, rmGM-CSF has a molecular mass of 14.3 kD analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques. |
| Source | E. coli |
| Biological Activity | ED50 < 5 pg/ml, measured in a cell proliferation assay using mouse FDC-P1 cells, corresponding to a specific activity of >2 x 108 units/mg. |
| Sequence |
Ala18-Lys141 (Accession #: Q14AD9), with an N-terminal Met.
|
Properties
| Measured Molecular Weight | 14.3 kDa, observed by reducing SDS-PAGE. |
| Purity | > 98% as analyzed by SDS-PAGE&HPLC. |
| Formulation | Lyophilized after extensive dialysis against PBS. |
| Reconstitution | Reconstituted in ddH2O or PBS at 100 μg/ml. |
| Endotoxin Level | < 0.2 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant Mouse GM-CSF remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Mouse GM-CSF should be stable up to 1 week at 4°C or up to 3 months at -20°C. |
| Note | For research use only |