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GM-CSF, Mouse

SKU: IX03300

GM-CSF, Mouse

SKU: IX03300
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Starting at: $82.00

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IX03300-11mgStarting at: $1,872.00
IX03300-1010ugStarting at: $82.00
IX03300-5050ugStarting at: $245.00

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Synonyms GMCSF,Granulocyte/Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, pluripoietin-alpha
Description Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine and other immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor that activates effector functions of granulocytes, monocytes/macrophages and eosinophils.
Recombinant Mouse GM-CSF produced in E.coli is a single non-glycosylated polypeptide chain containing 125 amino acids. A fully biologically active molecule, rmGM-CSF has a molecular mass of 14.3 kD analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.
Source E. coli
Biological Activity ED50 < 5 pg/ml, measured in a cell proliferation assay using mouse FDC-P1 cells, corresponding to a specific activity of >2 x 108 units/mg.
Ala18-Lys141 (Accession #: Q14AD9), with an N-terminal Met.


Measured Molecular Weight 14.3 kDa, observed by reducing SDS-PAGE.
Purity > 98% as analyzed by SDS-PAGE&HPLC.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant Mouse GM-CSF remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Mouse GM-CSF should be stable up to 1 week at 4°C or up to 3 months at -20°C.
Note For research use only