*** Product available by custom isolation only, please contact us to get a quote. ***
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of the easiest types of cells to grow in culture. Their durability also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen . There is evidence showing that fibroblasts in various organs are intrinsically different . Dermal fibroblasts switch from a proliferative, migratory phase to a contractile, matrix-remodeling phase during wound healing. In addition, they secrete large quantities of hyaluronan in response to inflammatory stimuli .
iXCells Biotechnologies provides high quality Human Dermal Fibroblasts-fetal (HDF-f), which are isolated from fetal human skin and cryopreserved at P0, with >0.5 million cells in each vial. HDF-f are characterized by their spindle morphology and express fibronectin. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand for 16 population doublings in Fibroblast Growth Medium (Cat# MD-0011) under the condition suggested by iXCells Biotechnologies.
|Tissue||Fetal human skin|
|Package Size||0.5 million cells/vial|
|Media||Fibroblast Growth Medium (Cat# MD-0011)|
 Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137.
 Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair and Regeneration (L. E. Glynn, ed.), pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
 Stair S, Carlson KW, Shuster S, Wei ET, Stern R (2002) Mystixin peptides reduce hyaluronan deposition and edema formation. Eur J Pharmacol 30;450(3):291-6.