Starting at: $49.00
| SKU | Package Size | Price | Quantity | Add to Cart |
|---|---|---|---|---|
| IX03017-1 | 1mg | Starting at: $157.00 | ||
| IX03017-10 | 10ug | Starting at: $49.00 | ||
| IX03017-50 | 50ug | Starting at: $95.00 |
Starting at: $699.00
Starting at: $1,012.00
Overview
| Synonyms | IGF-IA; Somatamedin C |
| Description | Insulin-like growth factor I (IGF-I) also known as Somatamedin C is a hormone similar in molecular structure to insulin. Human IGF-I has two isoforms (IGF-IA and IGF-IB) which is differentially expressed by various tissues. Mature human IGF-I respectively shares 94% and 96% aa sequence identity with mouse and rat IGF-I. Both IGF-I and IGF-II (another ligand of IGF) can signal through the IGF-I receptor (IGFIR), but IGF-II can alone bind the IGF-II receptor (IGFIIR/ Mannose-6-phosphate receptor). IGF-I plays an important role in childhood growth and continues to have anabolic effects in adults.
Recombinant human Insulin-like growth factor I (rhIGF-I) produced in E. coli is a single non-glycosylated polypeptide chain containing 70 amino acids. A fully biologically active molecule, rhIGF-I has a molecular mass of 7.7 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques. |
| Accession No | P05019 |
| Species | Human |
| Source | E. coli |
| Biological Activity | ED50 < 5 ng/ml, measured by a cell proliferation assay using FDC-P1 cells, corresponding to a specific activity of > 2.0× 105 units/mg. |
| Sequence |
GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ
TGIVDECCFR SCDLRRLEMY CAPLKPAKSA |
Properties
| Measured Molecular Weight | 7.7 kDa, observed by reducing SDS-PAGE. |
| Purity | > 95% by SDS-PAGE and HPLC analyses. |
| Formulation | Lyophilized after extensive dialysis against PBS. |
| Reconstitution | Reconstituted in ddH2O at 100 μg/ml. |
| Endotoxin Level | < 0.2 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant human Insulin-like growth factor I (rhIGF-I) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhIGF-I should be stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
| Note | For research use only |