Starting at: $107.00
| SKU | Package Size | Price | Quantity | Add to Cart |
|---|---|---|---|---|
| IX03076-1 | 1mg | Starting at: $2,621.00 | ||
| IX03076-10 | 10ug | Starting at: $107.00 | ||
| IX03076-50 | 50ug | Starting at: $344.00 |
Starting at: $699.00
Starting at: $1,012.00
Overview
| Synonyms | Za11 |
| Description | Interleukin-21 (IL-21) belongs to the Type I four helix bundle cytokines, and shares the common cytokine receptor γ chain with IL-2, IL-4, IL-7, IL-9, and IL-15. IL-21 is expressed by CD4+ T cells, natural killer (NK) T cells, and Th17 cells, and the IL-21 receptor is highly expressed on CD4+ and CD8+ B cells; indeed, IL-21 plays a pivotal role in the survival and proliferation of B cells, and their differentiation to immunoglobulin (Ig) producing cells. IL-21 up-regulates and down-regulates the production of IgG1 and IgE by B cells, respectively, and diminishes the severity of allergy and asthma. In some case, IL-21 also induces the apoptosis of B cells. The other roles of IL-21 include regulation of innate immune systems, implication on autoimmunity, and antitumor actions. Recombinant human Interleukin-21 (rhIL-21) produced in E. coli is a single non-glycosylated polypeptide chain containing 132 amino acids. A fully biologically active molecule, rhIL-21 has a molecular mass of 15.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques. |
| Accession No | Q9HBE4 |
| Species | Human |
| Source | E. coli |
| Biological Activity | ED50 < 0.5 ng/mL, measured by a cell growth inhibition assay using Mino cells, corresponding to a specific activity of > 2.0× 106 units/mg. |
| Sequence |
MQDRHMIRMR QLIDIVDQLK NYVNDLVPEF LPAPEDVETN
CEWSAFSCFQ KAQLKSANTG NNERIINVSI KKLKRKPPST NAGRRQKHRL TCPSCDSYEK KPPKEFLERF KSLLQKMIHQ HLSSRTHGSE DS |
Properties
| Measured Molecular Weight | 15.4 kDa, observed by reducing SDS-PAGE. |
| Purity | > 95% by SDS-PAGE analysis. |
| Formulation | Lyophilized after extensive dialysis against PBS. |
| Reconstitution | Reconstituted in ddH2O at 100 μg/mL. |
| Endotoxin Level | < 0.2 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant human Interleukin-21 (rhIL-21) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhIL-21 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
| Note | For research use only |