Starting at: $95.00
| SKU | Package Size | Price | Quantity | Add to Cart |
|---|---|---|---|---|
| IX02991-1 | 1mg | Starting at: $1,498.00 | ||
| IX02991-10 | 10ug | Starting at: $95.00 | ||
| IX02991-50 | 50ug | Starting at: $174.00 |
Starting at: $699.00
Starting at: $1,012.00
Overview
| Synonyms | MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor |
| Description | Interleukin-3 (IL-3) is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine. Recombinant human interleukin 3 expressed in CHO cells is glycosylated protein with molecular weight range from 17 to 30 kDa shown in non-reducing SDS-PAGE. |
| Species | Human |
| Source | CHO |
| Biological Activity | ED50 < 0.1 ng/ml, measured in a cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 1×107 units/mg. |
| Sequence |
APMTQTTPLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL
NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF |
Properties
| Measured Molecular Weight | 17-30 kDa, observed by non-reducing SDS-PAGE. |
| Purity | > 95% as analyzed by SDS-PAGE and HPLC. |
| Formulation | Lyophilized after extensive dialysis against PBS. |
| Reconstitution | Reconstituted in ddH2O or PBS at 100 μg/ml. |
| Endotoxin Level | < 0.2 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant human Interlerkin 3 (IL-3) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhIL-3 should be stable up to 1 week at 4°C or up to 2 months at -20°C. |
| Note | For research use only |