|Synonyms||Fibroblast Growth Factor-7, HBGF-7|
|Description||Keratinocyte Growth Factor (KGF) is a highly specific epithelial mitogen produced by fibroblasts and mesenchymal stem cells. KGF belongs to the heparin binding Fibroblast Growth Factor (FGF) family, and is known as FGF-7. However, in contrast to FGF-1, which binds to all known FGF receptors with high affinity, KGF only binds to a splice variant of the FGF receptor, FGFR2-IIIb. FGFR2-IIIb is expressedby most epithelial cells, indicating KGF’s roleas a paracrine mediator. KGF induces the differentiation and proliferation of various epithelial cells such as keratinocytes in the epidermis, hair follicles and sebaceous glands., KGF is also responsible for wound repair of various tissuesincluding lung, bladder, and kidney.
Recombinant mouse Keratinocyte Growth Factor (rmKGF) produced in E. coli is a single non-glycosylated polypeptide chain containing 163 amino acids. A fully biologically active molecule, rmKGF has a molecular mass of 18.7 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques.
|Biological Activity||ED50 < 2 ng/ml, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 5.0× 105 units/mg.|
CNDMSPEQTA TSVNCSSPER HTRSYDYMEG GDIRVRRLFC
RTQWYLRIDK RGKVKGTQEM KNSYNIMEIR TVAVGIVAIK
GVESEYYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY
ASAKWTHSGG EMFVALNQKG IPVKGKKTKK EQKTAHFLPM
|Measured Molecular Weight||18.7 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE and HPLC analyses.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in PBS at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant mouse Keratinocyte Growth Factor (rmKGF) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rmKGF remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.|
|Note||For research use only|