Excitation and inhibition are the two basic interactions between neurons, which utilize glutamate and γ-aminobutyric acid (GABA) as the major neurotransmitters for excitatory and inhibitory signals, respectively. Abnormal GABAergic neuron function has been associated with multiple neurodevelopmental and neurodegenerative disorders.
iXCells™ Cortical GABAergic Neurons show high neuronal purity (>90% Tuj1 positive cells) and express typical marker GABA (Figure 1 and Figure 2) after culturing in the Cortical Neuron Maintenance Medium (Cat# MD-0093) for 5 days.
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Figure 1. Phase contrast image of iPSC-GABAergic neurons cultured in the Cortical Neuron Maintenance Medium (Cat# MD-0093) for 5 days.
Figure 2. The cryopreserved GABAergic neurons were recovered and seeded on Matrigel-coated 96-well plate and cultured in the Cortical Neuron Maintenance Medium (Cat# MD-0093) for 5 days. The cells were fixed and stained with anti-GABA (red) and anti-TUJ1 (green) antibodies.
|Tissue Origin||Human iPSC-derived GABAergic Neurons|
|Package Size||1.0 million cells/vial (cryopreserved)
4.0 million cells/vial (cryopreserved)
|Shipped||Frozen on dry ice|
|Media||Cortical Neuron Maintenance Medium (Cat# MD-0093)|
 Yang N, Chanda S et al. (2017). “Generation of pure GABAergic neurons by transcription factor programming”. Nat Methods. 14(6):621-628.