Sensory neurons play a crucial role in the detection and response to different types of sensory stimuli, such as touch, temperature, and pain. As one of the most significant neuronal subtypes in the human peripheral nervous system, they form neuronal-glial networks that are responsible for a variety of motor and sensory mediated functions . Dysfunction of sensory neurons can lead to various neurological disorders such as pain, illnesses of the sensory neurons, amyotrophic lateral sclerosis (ALS), and problems with mechano- or temperature perception loss . Understanding the biology of iPSC-derived sensory neurons can help with new treatments for these disorders, as well as improve our understanding of normal sensory function .
iXCells Biotechnologies is proud to provide fully differentiated and functional human iPSC-derived human sensory neurons that display typical neuronal morphology and express key markers of e.g.,Peripherin, BRN3A (Figure 1) when cultured in the Human Sensory Neuron Maintenance Medium (Cat# MD-0114-100ML). In addition, our iPSC-derived sensory neurons can also be co-cultured with glia or other cell types for drug screening platforms.
Figure 1. Human iPSCs-derived sensory neurons show expression of characteristic biological markers.(A) Immunostaining of iPSC-derived astrocytes expressing MAP2 (green), BRN3A (red), Peripherin (red) 7 days post-thaw. All cells were counterstained for DAPI (blue). Scale bars, 200 µm. (B) Example raster plot showing spikes (black lines) and network bursts (pink lines) at different temperatures in the MEA for sensory neurons grown for two weeks. (C) The electrical parameters were measured in the MEA over different temperatures and the results are graphed as mean ± SEM for mean firing rate. (D) Gene expression values for NTRK1 (TRKA), TRPV1, SCN9A (Nav1.7) and SCN10A (Nav1.8) from three weeks post-thaw sensory neurons and control (iPSC-derived Astrocytes). Results are graphed as mean ± SEMs.
|Tissue||Human iPSC-derived sensory neurons (Normal)|
|Package Size||1.0 million cells/vial; 2.0 million cells/vial;|
|Media||Human Sensory Neuron Maintenance Medium (Cat# MD-0114-100ML)
Recovery Supplement (Cat# MD-0110-20μL)
Y27632 (Cat# MD-0025-2MG)
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 Lampert A, Bennett DL, McDermott LA, Neureiter A, Eberhardt E, Winner B, Zenke M. Human sensory neurons derived from pluripotent stem cells for disease modelling and personalized medicine. Neurobiol Pain. 2020 18;8:100055.