Fibroblasts and myofibroblasts are believed to be the key effector cells in renal fibrogenesis responsible for the synthesis and deposition of extracellular matrix components . Although fibroblasts are histologically visible in normal kidneys, there are relatively few of them and proximal tubular epithelial cells predominate. In progressive disease, however, the interstitium becomes filled with myofibroblasts . Fibroblasts are considered the primary matrix-producing cells in the kidney and hence they are clinically relevant as principal mediators of renal fibrosis associated with progressive renal failure .
iXCells Biotechnologies provides high quality Human Renal Fibroblasts, which are isolated from adult human lung tissue and cryopreserved at P1, with >0.5 million cells in each vial. Human Renal Fibroblasts express fibronectin and are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. They can further expand no more than 3 passages in Fibroblast Growth Medium (Cat# MD-0011) under the condition suggested by iXCells Biotechnologies.
Figure 1. Human Renal Fibroblasts. (A) Phase contrast image. (B) Immunofluorescence staining with antibodies against Vimentin.
|Tissue||Normal human kidney tissue|
|Package Size||0.5 million cells/vial|
|Media||Fibroblast Growth Medium (Cat# MD-0011)|
 Soma Meran and Robert Steadman (2011) “Fibroblasts and myofibroblasts in renal fibrosis”. Int. J. Exp. Path. 92: 158–167
 H. Terence Cook. (2010) “The Origin of Renal Fibroblasts and Progression of Kidney Disease”. The American Journal of Pathology, 176 (1):22-24.
 Frank Strutz and Michael Zeisberg. (2002) “Renal Fibroblasts and Myofibroblasts in Chronic Kidney Disease”. JASN, 17: 2992-2998.