Epidermal keratinocyte is the predominant cell type in the outermost layer of the skin – the epidermis, which serves as a critical barrier to separate and protect the inside of human body from outside environment and damage from pathogens, heat, UV radiation and water loss. Epidermal keratinocytes originate in the stratum basale, and they undergo gradual differentiation and migrate towards the surface of the epidermis until they reach the stratum corneum, where they form a tight layer of nucleus-free and highly keratinized squamous cells. This layer forms an effective barrier to prevent water loss and the entry of infectious agents. Keratinocytes are also known to produce various growth factors, cytokines, antimicrobial peptides, and complement factors. Therefore, keratinocytes are important for wound healing, inflammation, infection, skin microbiome and immune response.
iXCells Biotechnologies provides high quality primary Mouse Epidermal Keratinocytes-neonatal (MEK-n), which are isolated from neonatal mouse skin and cryopreserved at P0, with >0.5 million or >1 million cells in each vial.MEK-n are characterized by phalloidin staining and are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. MEK-n are not recommended for expanding or long term cultures since the cells do not proliferate in culture.
Figure 1. (A). Phase contrast image of primary mouse epidermal keratinocytes-neonatal (MEK-n). (B). MEK-n were differentiated in the presence of 0.2 mM CaCl2, and cells were stained with phalloidin (red) to visualize the formation of actin fiber-rich filopodial projections between adjacent cells during differentiation. Nuclei were counterstained with DAPI.
|Tissue||Epidermis from mouse neonatal skin|
|Package Size||1 million cells/vial (Enough to seed on four wells of 6-well plate or eight wells of 12-well plate, and cells will reach 80% confluency in 5 days for assay).
|Media||Keratinocyte Growth Medium (Cat# MD-0047)
Mouse Keratinocyte Differentiation Medium (Cat# MD-0062)
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 Pasparakis M, Haase I, Nestle FO. Nat Rev Immunol. 2014 May;14(5):289-301. Mechanisms regulating skin immunity and inflammation.