Podocytes Culture Medium

SKU	Package Size	Price	Quantity	Add to Cart
MD-0120-500mL	500 ML	Starting at: $267.00	Podocytes Culture Medium quantity

Description

Product Description

Podocytes are terminally differentiated highly specialized epithelial cells that lining the outer surface of the glomerular capillaries in kidney ^[1]. Podocytes together with the opposing monolayers of fenestrated endothelial cells in the vascular space and glomerular basement membrane (GBM) in between form the three-layer glomerular filtration barrier which functions as size-selective and charge-dependent molecular sieve for the main function of nephron. Podocytes injury, which is usually termed “foot process effacement” is a pathological process found in many kidney diseases ^[2]. In vitro cultured podocytes are useful model to study podocytes biology. These cells can also be used with glomerular endothelial cells for coculture studies in an attempt to better study barrier permeability or be cultured in Matrigel for three-dimensional or organoid studies^[3].

iXCells^® Podocytes Culture Medium is a complete medium uniquely designed to maintain primary podocyte growth in vitro. The cells maintain their cell specific markers as well as function for as long as 2-3 passages which makes it suitable for the following cell characterization and functional assays. The medium contains all the necessary nutrients that podocyte needs to grow and perform their function. The medium is provided as the antibiotics free complete culture medium supplemented with the working concentration of fetal bovine serum (FBS). No additional FBS is needed for cell culture.

Product Details

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SKU

MD-0120

Country of manufacture

United States of America (USA)

Quantity

One Bottle

Form

Liquid

Component, Package and Storage

Component	Name	Size	Shipping	Storage
MD-0120	Podocytes Culture Medium	500 mL	Dry ice	-20°C

Note: Please store the medium immediately at -20°C upon arrival.

Sterility Test

Negative for bacteria, yeast, fungi, and mycoplasma.

Product line

Mammalian cells
Primary cells
With serum (Fetal bovine serum)
With phenol red
Antibiotics free

Product use

Do not use components that are beyond the expiration date indicated on the label.
Tested in the iXCells Human Podocytes (Cat: 10HU-250) following the recommended protocol in a 37 °C, 5% CO2 incubator.
For laboratory research use only. Not for use in Diagnostic procedures.
Results may vary due to variations among species, organ or tissue origin, and donors.
Results may vary due to different culture conditions including temperature, CO2 concentration, culture vessels, coating conditions, medium adding amount, and medium change frequence. For certain culture or test conditions, please follow the culture protocol for each specific cell type.
It is not approved for human or animal use.
iXCells in house cell culture test was performed under antibiotics free condition for ≥ 3 days by following the recommended protocol, and no bacterial or fungi contamination were observed during the culture period.
Optional: Antibiotics-Antimycotic (Fisher, Cat# 15240062) or Penicillin Streptomycin (Thermo Fisher, Cat# 15140122) at working solution could be added if necessary, which have been tested in house without visible effect on cell morphology or function.

Precaution

When handling biohazardous materials such as cryopreserved cells, safe laboratory procedures should be followed, and personal protective equipment should be worn including but not limited to face masks, gloves, and lab coat.

Datasheet

Fully supplemented medium: No extra fetal bovine serum is needed for cell culture.
Antibiotics free: Antibiotics-Antimycotic (Fisher, Cat# 15240062) or Penicillin-Streptomycin (Thermo Fisher, Cat# 15140122) may be added at the working solution.
Long-Term Culturing Viability: Following iXCells optimized condition, the Human Podocytes (Cat#10HU-250) can be subcultured for additional 2-3 passages, maintaining their functionality and morphology.