Adipose tissue is a major metabolic organ, and it has been traditionally classified as either white adipose (WAT) or brown adipose tissue (BAT) . Adipose-derived stem cells are multipotent mesenchymal stem cells (MSCs) that are capable of differentiating into adipocytes, osteocytes, chondrocytes etc. in vitro. They have been applied in studies such as stem cell differentiation, regenerative medicine , cell therapy, tissue engineering and creation of iPS cell lines. Adipose-derived stem cells (ADSCs) are an attractive source of material for mesenchymal stem cell research due to the abundance of adipose and relative ease of access compared with bone marrow .
iXCells Biotechnologies provides high quality Rat Adipose Derived Stem Cells (rADSC), which are isolated from inguinal white fat tissue (Cat# 10RA-001) or interscapular brown fat tissue (Cat# 10RA-002). The cells were cryopreserved at P1, with ≥0.5 million cells in each vial. The characterization was performed by CD29 staining (Figure 1) and lipid staining after differentiation (Figure 2). rADSC are negative for mycoplasma, bacteria, yeast, and fungi and can be cultured no more than 3 passages using Adipose-derived Stem Cells Growth Medium (Cat# MD-0003) under the condition suggested by iXCells Biotechnologies. In vitro, rADSCs can be differentiated into adipocytes and osteoblasts using Adipocyte Differentiation Medium (Cat# MD-0005) and Osteogenic Differentiation Medium (Cat# MD-0006) (Figure 2), separately. Extensive expansion was not recommended because the cells may lose their multipotent properties.
Figure 1. (A) Rat ADSCs-white fat (phase contrast). (B) Rat ADSCs-white fat ICC staining using CD29 antibody.
Figure 2. (A): Rat ADSCs-Brown fat adipocyte differentiation (Day 22 post adipogenic induction). (B) Rat ADSCs-Brown fat osteogenic induction (Day 24 post osteogenic induction)
|Tissue||Sprague-Dawley rat interscapular brown fat tissue|
|Package Size||0.5 millioncells/vial|
|Media||Adipose-derived Stem Cells Growth Medium (Cat# MD-0003)
Adipocyte Differentiation Medium (Cat# MD-0005)
Osteogenic Differentiation Medium (Cat# MD-0006)
 Anna Park, Won Kon Kim, and Kwang-Hee Bae. (2014) Distinction of white, beige and brown adipocytes derived from mesenchymal stem cells. World J Stem Cells. 6(1): 33-42.
 Harasymiak-Krzyzanowska I et al. (2013) Adipose tissue-derived stem cells show considerable promise for regenerative medicine applications. Cell Mol Biol Lett. 18(4): 479-493.