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Mouse stromal vascular fraction – brown fat (MSVF-bf)

SKU: 10MU-008

Mouse stromal vascular fraction – brown fat (MSVF-bf)

SKU: 10MU-008
Pricing Starting at

Starting at: $723.00

Available Options

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10MU-008Cryopreserved, 1.0 million cells/vialStarting at: $723.00

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Description

Product Description

Mouse stromal vascular fraction (SVF) is freshly isolated heterogeneous cell fraction derived from the brown adipose tissue (BAT, brown fat) or white adipose tissue (white fat) [1]. Although not a fully defined cell population, the SVF includes vascular smooth muscle cells, fibroblasts, mast cells, macrophages, lymphocytes, endothelial cells, preadipocytes, and adipose-derived stromal/stem cells (ASCs). There is an increasing interest in the biology and therapeutic potential of SVF because of the direct and rapid isolation procedure in a xenobiotic-free environment [2].

iXCells Biotechnologies provides high quality Mouse stromal vascular fraction (SVF), which are isolated from inguinal white fat tissue (Cat# 10MU-009) or interscapular brown fat tissue (Cat# 10MU-008). The cells were cryopreserved at P0, with ≥1.0 million cells in each vial. The characterization was performed by culturing the cells using Adipose-derived Stem Cells Growth Medium (Cat# MD-0003) followed by CD29 staining (Figure 1). mSVF is negative for mycoplasma, bacteria, yeast, and fungi and can be cultured no more than 3 passages using Adipose-derived Stem Cells Growth Medium (Cat# MD-0003) under the condition suggested by iXCells Biotechnologies. Extensive expansion was not recommended because the cells may lose their multipotent properties.

datasheet image

Figure 1. mSVF(white fat) was recovered and cultured using Adipose-derived Stem Cells Growth Medium (Cat#MD-0003) for 7 days. (A) Phase contrast image. (B) ICC staining using CD29 antibody.

Product Details

Tissue

C57BL/6 or BALB/C mice interscapular brown fat tissue

Package Size

1.0million cells/vial

Passage Number

P0

Shipped

Cryopreserved

Storage

Liquid nitrogen

Growth Properties

Adherent

Media

Adipose-Derived Stem Cells Growth Medium (Cat# MD-0003)

 

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