Mouse stromal vascular fraction (SVF) is freshly isolated heterogeneous cell fraction derived from the brown adipose tissue (BAT, brown fat) or white adipose tissue (white fat) . Although not a fully defined cell population, the SVF includes vascular smooth muscle cells, fibroblasts, mast cells, macrophages, lymphocytes, endothelial cells, preadipocytes, and adipose-derived stromal/stem cells (ASCs). There is an increasing interest in the biology and therapeutic potential of SVF because of the direct and rapid isolation procedure in a xenobiotic-free environment .
iXCells Biotechnologies provides high quality Mouse stromal vascular fraction (SVF), which are isolated from inguinal white fat tissue (Cat# 10MU-009) or interscapular brown fat tissue (Cat# 10MU-008). The cells were cryopreserved at P0, with ≥1.0 million cells in each vial. The characterization was performed by culturing the cells using Adipose-derived Stem Cells Growth Medium (Cat# MD-0003) followed by CD29 staining (Figure 1). mSVF is negative for mycoplasma, bacteria, yeast, and fungi and can be cultured no more than 3 passages using Adipose-derived Stem Cells Growth Medium (Cat# MD-0003) under the condition suggested by iXCells Biotechnologies. Extensive expansion was not recommended because the cells may lose their multipotent properties.
Figure 1. mSVF(white fat) was recovered and cultured using Adipose-derived Stem Cells Growth Medium (Cat#MD-0003) for 7 days. (A) Phase contrast image. (B) ICC staining using CD29 antibody.
|Tissue||C57BL/6 or BALB/C mice inguinal white fat tissue|
|Package Size||1.0 million cells/vial|
|Media||Adipose-Derived Stem Cells Growth Medium (Cat# MD-0003)|