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Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their durability also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen . There is evidence showing that fibroblasts in various organs are intrinsically different . In the lymphoid compartment, fibroblasts construct a unique conduit system with intriguing size exclusion which plays a fundamental role in regulating immune response and homeostasis of tissue fluids .
iXCells Biotechnologies provides high quality Rat Lymphatic Fibroblasts (RLF), which are isolated from rat lymph node and cryopreserved at P0, with >0.5 million cells in each vial. RLF are characterized by their spindle-shaped morphology. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand for 5 population doublings in Fibroblast Growth Medium (Cat# MD-0011) under the condition suggested by iXCells Biotechnologies.
|Tissue||Rat lymph node|
|Package Size||0.5 million cells/vial|
|Media||Fibroblast Growth Medium (Cat# MD-0011)|
 Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137.
 Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair and Regeneration (L. E. Glynn, ed.), pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981.
 Domracheva, E. V., Rogova, E. M., Vorob’ev, A. L. (1984) Monolayer culture of bone marrow and splenic fibroblasts in lymphoproliferative diseases. Ter Avkh. 56:63-65.