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Chondrocytes are the resident cells of cartilage and are responsible for synthesizing a range of collagenous and non-collagenous extracellular matrix macromolecules. These include collagen type II, aggrecan, link protein, collagen type IX, and collagen type XI . The control of proliferation and differentiation of chondrogenic cells is central to the coordinated development of the vertebrate skeleton. Chondrocytes are capable of producing and responding to a large number of peptide growth factors and cytokines, including insulin-like growth factor-1 and interleukin-1 . Chondrocyte cultures are useful in vitro models for studying cartilage regeneration and repair, cytokine and growth factor effects on cartilage, specific gene regulation and pathophysiology of arthritis.
iXCells Biotechnologies provides high quality Human Chondrocytes-articular (HC-a), which are isolated from human articular cartilage and cryopreserved at P0, with >0.5 million cells in each vial. These HC-a express S100 and type II collagen. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungiand can further expand for 12 population doublings in Chondrocyte Medium (Cat# MD-0040) under the condition suggested by iXCells Biotechnologies.
|Tissue||Human articular cartilage|
|Package Size||0.5 millioncells/vial|
|Media||Chondrocyte Medium (Cat# MD-0040)|
. E. Kolettas, H. I. Muir, J. C. Barrett 1 and T. E. Hardingham (2001) Chondrocyte phenotype and cell survival are regulated by culture conditions and by specific cytokines through the expression of Sox-9 transcription factor. Rheumatology, 40(10): 1146-1156.
. Fosang A, Tyler JA, Hardingham TE, (1991) Effect of interleukin-1 and insulin-like growth factor-1 on the release of proteoglycan components and hyaluronan from pig articular cartilage in explant culture. Matrix 11:17-24.