10HU-256
Starting at:
Starting at: $1,020.00
Starting at: $1,020.00
| SKU | Package Size | Price | Quantity | Add to Cart |
|---|---|---|---|---|
| 10HU-256-0.5M | Cryopreserved, 0.5 million cells/vial | Starting at: $1,020.00 |
Starting at: $1,020.00
Starting at: $986.00
Product Description
Synovial joints are unique functional elements of the body and provide the ability for locomotion and for physical interaction with the environment[1,2]. The inner lining of the joint consists of the synovium, also called the synovial membrane, a thin layer located between the joint capsule and the joint cavity. Fibroblast-like synoviocytes (FLS) are mesenchymal origin cells located inside the synovial membrane. FLS display many characteristics common with fibroblasts, such as expression of several types of collagens and protein vimentin, a part of cytoskeletal filament. Once activated, FLS are suggested to acquire a myofibroblast-like phenotype that drives fibrogenesis through excessive extracellular matrix component deposition and an enhanced contractile function. These cells play a crucial role in the pathogenesis of chronic inflammatory diseases, such as rheumatoid arthritis. Therefore, Human FLS are an important model to understand articular function in degenerative and inflammatory joint diseases[1-3].
iXCells Biotechnologies provides high quality Human Fibroblast-Like Synoviocytes- Osteoarthritis (HFLS-OA), which are isolated from osteoarthritic human synovial membrane and cryopreserved at P2, with ≥0.5 million cells in each vial. HFLS-OA express fibronectin and vimentin and are characterized by their spindle-shaped morphology. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can be further expanded for no more than 3 passages in Synoviocyte Growth Medium (Cat# MD-0056) under the conditions suggested by iXCells Biotechnologies.
Figure 1. Human Fibroblast-Like Synoviocytes-Osteoarthritis (HFLS-OA). The cells were recovered, and seeded at 10,000 cells/cm2 following iXCells’ protocol. Phase contrast images were taken at the indicated time post recovery.
Figure 2. Immunofluorescence staining with antibodies against Vimentin (green) and Fibronectin (red) of Human Fibroblast-Like Synoviocytes–Osteoarthritis (HFLS-OA).
Product Details
| Tissue | Human synovial membrane |
| Package Size | 0.5 million cells/vial |
| Passage Number | P2 |
| Shipped | Cryopreserved |
| Storage | Liquid nitrogen |
| Growth Properties | Adherent |
| Media | Synoviocyte Growth Medium (Cat# MD-0056) |
Starting at: $1,020.00
Starting at: $986.00
FREQUENTLY BOUGHT TOGETHER - RECOMMENDED FOR YOU!
Starting at: $1,020.00
Starting at: $986.00