Y27632 is a cell-permeable small molecule Rho-associated kinase (ROCK) inhibitor . Y27632 has been found to prevent apoptosis as well as enhance the survival and cloning efficiency of dissociated human embryonic stem (ES) cells without affecting their self-renewal properties or pluripotency . This molecule has also been shown to enhance survival during the transplantation of ES cell-derived neural precursors . Y27632 in combination with Pifithrin-µ significantly improves cell recovery after cryopreservation .
|Quantity||2mg (10mM in 624.4μl DMSO)|
|Alternate Name||(1R,4r)-4-((R)-1-aminoethyl)-N-(pyridine-4-yl)cyclohexanecarboxamide dihydrochloride|
|Molecular Weight||320.3 g/mol|
|Purity||>Greater than 98% by HPLC analysis|
|Applications||MAINTENANCE AND SELF-RENEWAL
|Product Use||Y27632 is for research use only. It is not approved for human or animal use, or for application in vitro diagnostic procedures.|
|Storage & stability||Store aliquots at -20°C. Stock solutions are stable for 6 months when stored as directed.|
 Ishizaki, T., Uehata, M., Tamechika, I., Keel, J., Nonomura, K., Maekawa, M., and Narumiya, S. (2000) Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol 57: 976-983.
 Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J.B., Nishikawa, S., Nishikawa, S., Muguruma, K., and Sasai, Y. (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells.Nat Biotechnol 25: 681-686.
 Koyanagi, M., Takahashi, J., Arakawa, Y., Doi, D., Fukuda, H., Hayashi, H., Narumiya, S., and Hashimoto, N. (2008) Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors.J. Neurosci Res. 86: 270-280.
 Xu, X., Cowley, S., Flaim, C.J., James, W., Seymour, L.W., Cui, Z. (2010) Enhancement of cell recovery for dissociated human embryonic stem cells after cryopreservation. Biotechnol Prog. 26: 781-788.