CUSTOM CELL ISOLATION SERVICES

(Top) Freshly or cryopreserved human monocytes (positive or untouched) were induced to differentiate into dendritic cells (DCs) following iXCells protocol. (Bottom) PBMCs or purified Pan T cells isolated from the corresponding donors were gently added into the wells containing DCs. 24 hours post incubation, the supernatant was collected, and performed for IFN  MSD assay. The IFN  release was shown by the IFN concentration calculated using the standard curve after background subtraction.

(Top) Freshly or cryopreserved human monocytes (positive or untouched) were induced to differentiate into dendritic cells (DCs) following iXCells protocol. (Bottom) PBMCs or purified Pan T cells isolated from the corresponding donors were gently added into the wells containing DCs. 24 hours post incubation, the supernatant was collected, and performed for IFNγ MSD assay. The IFNγ release was shown by the IFNγ concentration calculated using the standard curve after background subtraction.

Freshly isolated human neutrophils (Above) were incubated with the human epidermoid carcinoma cell line A431 which was stably labeled with Nuclight Red according to the various E: T ratio. The A431-Nuclight Red cell growth was monitored using InCucyte shown as Red Objective count. (Bottom Left) Representative images taken by Essence InCucyte at 72 hours co-culture; (Bottom Right) The statistic analysis of A431-Nuclight Red cell growth with or without neutrophils co-culturing at various E:T ratio.

(Top and Middle) Fresh or cryopreserved PBMCs labeled with CFSE were incubated with CD3 and CD28 antibodies. The proliferation of PBMCs were measured by decrease of the fluorescence using flow cytometry analysis. (Bottom) Human lung carcinoma cells A549 stably labeled with Nuclight Red were incubated with PBMCs, with or without the activation. The A549-Nuclight Red cell growth was monitored using InCucyte shown as Red Objective count. iXCells has set up the platform to screen the compounds which may enhance or inhibit the PBMC killing capability.

(Top and Middle) Freshly isolated human pan T cells labeled with CFSE were activated by incubating with CD3 and CD28 antibodies. The proliferation of pan T cells were measured by decrease of the fluorescence using flow cytometry analysis. (Bottom) Human lung carcinoma cells A549 stably labeled with Nuclight Red were incubated with the Pan T cells, isolated from 2 unique donors. The A549-Nuclight Red cell growth was monitored using Incucyte shown as Red Objective count. iXCells has set up the platform to screen the compounds which may enhance or inhibit the T cell killing capability.