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Human iPS Cell Line (SOD1 mutation, A4V, HOM)

SKU: 30HU-101

Human iPS Cell Line (SOD1 mutation, A4V, HOM)

SKU: 30HU-101
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Description

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Product Description

Human iPSC line with SOD1 mutation at A4V.

Human iPSC markers

Figure 1. iXCells human iPS cells are characterized by immunostaining with Oct4, Nanog, Sox2, SSEA4, TRA-1-60-R, TRA-1-81.

Human iPSC immunostaining

Figure 2. (A) iXCells human iPS cells are positive for the transcription factor Oct4 (green) and the surface marker SSEA4 (red). (B) iXCells human iPS cells are positive for the surface markers TRA-1-81 (green) and SSEA4 (red).

Teratoma formation

Figure 3.In vivo validation of iXCells human iPS cells by teratoma formation.

Karyotyping of iPSC Line

Figure 4.Karyotyping of iPSC Line generate by iXCells Biotechnologies.

Product Description

Tissue Origin Human iPS Cells derived from dermal fibroblasts, adipose-derived stem cells, or peripheral blood mononuclear cells
Package Size 2 vials of homozygous SOD1-A4V iPSC &  1 vial of isogenic control
Shipped Cryopreserved
Storage Liquid nitrogen
Growth Properties Adherent
Media & Reagents Human iPSC Growth Medium (Cat# MD-0018)
MEF Conditioned Medium (Cat# MD-0015)
Human iPSC Feeder-Free Growth Medium (Cat# MD-0019)
Human iPSC Xeno-Free Growth Medium (Cat# 0074)
iMEF Feeder (CF1), irradiated (Cat# 10MU-001)

 

References

[1] Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong H, Nakagawa M, Tanabe K, Tezuka K, Shibata T, Kunisada T, Takahashi M, Takahashi J, Saji H, Yamanaka S. A more efficient method to generate integration-free human iPS cells. Nat Methods. 2011 May; 8(5):409-12.

 

Manuals & Protocols

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