Description
Overview
Synonyms | Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF |
Description | Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effectors functions of granulocytes, monocytes/macrophages and eosinophils. |
Species | Mouse |
Source | CHO |
Biological Activity | ED50 < 0.05 ng/ml, measured in a cell proliferation assay using mouse FDC-P1 cells, corresponding to a specific activity of >2 x 107 units/mg. |
Sequence |
APTRSPITVT RPWKHVEAIK EALNLLDDMP VTLNEEVEVV
SNEFSFKKLT CVQTRLKIFE QGLRGNFTKL KGALNMTASY YQTYCPPTPE TDCETQVTTY ADFIDSLKTF LTDIPFECKK PVQK |
Properties
Measured Molecular Weight | 15~19 kDa, observed by non-reducing SDS-PAGE. |
Purity | > 95% as analyzed by SDS-PAGE and HPLC. |
Formulation | Lyophilized after extensive dialysis against PBS. |
Reconstitution | Reconstituted in ddH2O or PBS at 100 μg/ml. |
Endotoxin Level | <0.2 EU/μg, determined by LAL method. |
Storage | Lyophilized recombinant murine Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rmGM-CSF should be stable up to 1 week at 4°C or up to 2 months at -20°C. |
Note | For research use only |