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Human Leydig Cells (HLC)

SKU: 10HU-103

Human Leydig Cells (HLC)

SKU: 10HU-103
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Product Description

Human leydig Cells (HLC) are located in the interstitium of the testis near the seminiferous tubules [1, 2]. One of the main functions of HLC is to produce testosterone after stimulation by pituitary luteinizing hormone [1, 2, 3]. HLC, through synthesis of testosterone, play a key role in mammalian sex determination by inducing differentiation of the Wolffian ducts into male reproductive organs [3]. Additionally, HLC produce insulin-like growth factor 3 which is required for scrotal descent of the testis [1]. Recent studies have indicated that molecules such as cyclic nucleotide phosphodiesterases can modulate testosterone production in HLC, suggesting that testosterone synthesis may be manipulated using pharmacological targeting [2]. Cultured HLC are a useful in vitro model to better understand testicular development and to develop treatments for male reproductive disorders (Med para Hombres).

iXCells Biotechnologies provides high quality HLC, which are isolated from human testis and cryopreserved at P1, with >0.5 million cells in each vial. HLC express cytokeratin-18, and GATA-4. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand for 5 population doublings in Epithelial Cell Growth Medium (Cat# MD-0041) under the condition suggested by iXCells Biotechnologies.

Product Details

Tissue Human testis
Package Size 0.5 million cells/vial
Passage Number P1
Shipped Cryopreserved
Storage Liquid nitrogen
Growth Properties Adherent
Media Epithelial Cell Growth Medium (Cat# MD-0041)


[1] Chen H, Ge R, Zirkin B. “Leydig cells: From stem cells to aging.” Mol Cell Endocrinol. 306(1-2): 9-16.

[2] Vasta V, Shimizu-Albergine M, Beavo J. “Modulation of Leydig cell function by cyclic nucleotide phosphodiesterase 8A.” Proc Natl Acad Scie USA. 103(52): 19925-19930.

[3] Lejeune H, Habert R, Saez JM. “Origin, proliferation and differentiation of Leydig cells.” J Mol Endocrinol. 20(1): 1-25.

  • Kotula-Balak, M., Gorowska-Wojtowicz, E., Milon, A., Pawlicki, P., Tworzydlo, W., Płachno, B. J., . . . Bilinska, B. (2020). Towards understanding leydigioma: Do g protein-coupled estrogen receptor and peroxisome proliferator–activated receptor regulate lipid metabolism and steroidogenesis in leydig cell tumors? Protoplasma, 257(4), 1149-1163. doi:10.1007/s00709-020-01488-y -- Learn More

  • Duliban, M., Gorowska-Wojtowicz, E., Tworzydlo, W., Rak, A., Brzoskwinia, M., Krakowska, I., . . . Bilinska, B. (2020). Interstitial leydig cell tumorigenesis—leptin and adiponectin signaling in relation to aromatase expression in the human testis. International Journal of Molecular Sciences, 21(10), 3649. doi:10.3390/ijms21103649 - Learn More
  • Strange, D. P., Jiyarom, B., Pourhabibi Zarandi, N., Xie, X., Baker, C., Sadri-Ardekani, H., . . . Verma, S. (2019). Axl promotes zika virus entry and modulates the antiviral state of human sertoli cells. MBio, 10(4). doi:10.1128/mbio.01372-19 -- Learn More
  • Gorowska-Wojtowicz, E., Duliban, M., Kudrycka, M., Dutka, P., Pawlicki, P., Milon, A., . . . Bilinska, B. (2019). Leydig cell tumorigenesis - implication of g-protein coupled membrane estrogen receptor, peroxisome proliferator-activated receptor and xenoestrogen exposure. in vivo and in vitro appraisal. Tissue and Cell, 61, 51-60. doi:10.1016/j.tice.2019.08.001 -- Learn More
  • Kotula-Balak, M., Gorowska-Wojtowicz, E., Milon, A., Pawlicki, P., Kaminska, A., Pardyak, L., . . . Wolski, J. K. (2018). Towards understanding biology of leydiogioma. g protein-coupled receptor and peroxisome proliferator-activated receptor crosstalk regulates lipid metabolism and steroidogenesis in leydig cell tumors. BioRxiv. doi:10.1101/477901 -- Learn More