Neural stem cells (NSCs) are self-renewing, multipotent cells that generate the main phenotype of the nervous system. They primarily differentiate into neurons, astrocytes, and oligodendrocytes . The recent discovery of induced pluripotent stem cells (iPSCs) not only overcomes the ethical and logistical issues associated with human embryonic stem cells, but also provides a flexible platform for generating various differentiated cell types from diseased individuals.iPSC-derived NSCs are a potentially valuable source of in vitro models for complex, polygenic human diseases, and are potentially useful for drug discovery and cell-based therapy applications .
iXCells Biotechnologies provides high quality human neural stem cells (NSCs) derived from normal or diseased iPS cell lines. These cells express typical markers of neural stem and progenitor cells, e.g. Nestin, Pax6 and Sox1 (Figure 1 and Figure 2), with the purity higher than 97% (Figure 3). The cells have been fully characterized for their self-renewal and multi-potency. The iPSC-derived NSCs can be differentiated into astrocytes or motor neurons (Figure 4). Cells can further expand for 3-5 passages in Human Neural Stem Cell Growth Medium (Cat# MD-0024), but they are not recommended for extensive expansion, because the purity of the neural stem cell population may decrease.
All the cells provided by iXCells are negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C. The basic donor information (gender / age / race) is provided for each cell lot purchased.
Figure 1. iPSC-derived NSCs express Nestin and Pax6.
Figure 2. iPSC-derived NSCs express Nestin and Sox1.
Figure 3. More than 97% of the NSCs are Nestin positive.
Figure 4. iPSC-derived NSCs can be differentiated into GFAP+ astrocyte (A) or HB9+motor neurons (B).
|Tissue||Human Neural Stem Cells Derived from iPSCs (Normal, diseased)|
|Package Size||2.0 millioncells/vial|
|Media||Human Neural Stem Cell Growth Medium (Cat# MD-0024)|
 Alenzi, F; Bahkali, A (2011). “Stem cells: Biology and clinical potential”.African Journal of Biotechnology10(86): 19929–40.
 Dolmetsch R, Geschwind DH. (2011) “The human brain in a dish: the promise ofiPSC-derivedneurons”. Cell. 145(6):831-4.