The pancreas is an endocrine gland and digestive organ which secretes hormones and produces pancreatic juice to aid in digestion[1,2].Pancreatic islets function as pancreatic endocrine cells to produce vital hormones such as glucagon, insulin, and amylin[1,2]. Hence, Human Pancreatic Microvascular Endothelial Cells (HPaMEC) play a critical role to support the islet by transporting oxygen and nutrients to the pancreas, and affecting beta ell function and proliferation, influencing insulin gene expression during islet development, and product various growth factors[1,2]. Additionally, HPaMEC are involved in optimizing blood glucose sensing and regulation. HPaMEC can be used an in vitro model for studying islet biology, pancreatic cancer, transplantation, and regenerative medicine.
iXCells Biotechnologies provides high quality HPaMEC, which are isolated from human pancreatic tissue and cryopreserved at P2 after purification, with ≥0.5 million cells in each vial. HPaMEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII, VE-Cadherin and/or CD31 (PECAM) (Figure 1). They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HPaMEC can proliferate in Endothelial Cell Growth Medium (Cat# MD-0010), but they are not recommended for further expansion, because the purity of the endothelial population may decrease.
Figure 1. Human Pancreatic Microvascular Endothelial Cells (HPaMEC). (A) Phase contrast image of HPaMEC. (B & C) Immunofluorescence staining with antibodies against VE-Cadherin (B) and vWF/Factor VIII (C).
|Tissue||Human pancreatic tissue|
|Package Size||0.5 x 10^6 cells/vial|
|Media||Endothelial Cell Growth Medium (Cat# MD-0010)|
 Zanone M, Favaro E, Camussi G (2008). “From endothelial to beta cells: insights into pancreatic islet microendothelium.” Curr Diabetes Rev. 4(1): 1-9. Zanone M, Favaro E, Camussi G (2008). “From endothelial to beta cells: insights into pancreatic islet microendothelium.” Curr Diabetes Rev. 4(1): 1-9.
 Konstantinova I and Lammert E. (2004). “Microvascular development: learning from pancreatic islets.” Bioessays, 26(10):1069-1075.