The pulmonary vasculature is of great physiological/pathological significance. Human Pulmonary Artery Endothelial Cells (HPAEC) are critical to maintain the function of the pulmonary circulation and effective diffusion capacity of the lung. HPAEC provide a useful tool for studying various aspects of pathology and biology of the lung vasculature in vitro .
iXCells Biotechnologies provides high quality HPAEC, which are isolated from human pulmonary artery from single donors and cryopreserved at P2, with >0.5 million cells in each vial. HPAEC express vWF/Factor VIII and CD31 (Figure 1) and are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. They can further expand in Endothelial Cell Growth Medium (Cat# MD-0010) under the condition suggested by iXCells Biotechnologies.
Figure 1. HPAEC provided by iXCells are positive for CD31 (A) and vWF (B), as shown by immunostaining. Flow analysis showed that more than 99% of cells are CD31 positive (A’) and more than 97% are vWF positive (B’).
|Tissue||Human pulmonary artery|
|Package Size||0.5 million cells/vial|
|Media||Endothelial Cell Medium Kit (Cat# MD-0010)|
 Meuchel LW, Thompson MA, Cassivi SD, Pabelick CM and Prakash YS. Neurotrophins induce nitric oxide generation in human pulmonary artery endothelial cells. Cardiovasc Res. 2011; 91(4): 668-676.